切片機(jī)是目前國內(nèi)研制生產(chǎn)的產(chǎn)品,它廣泛地應(yīng)用于科研、教學(xué)、化工、醫(yī)療衛(wèi)生等單位,專切新鮮的或經(jīng)過固定的動(dòng)、植物標(biāo)本,特別是新鮮的腦組織。
Slicer is a product developed and produced in our country. It is widely used in scientific research, teaching, chemical industry, medical and health units. It specially cuts fresh or fixed animal and plant specimens, especially fresh brain tissue.
切片機(jī)切片時(shí)組織標(biāo)本不需冰凍或包埋,為此,樣品片避免了冰晶破壞,還能保持樣品活性和細(xì)胞良好形態(tài),給“免疫細(xì)胞化學(xué)研究 ”以及“脊髓和腦薄片的神經(jīng)生物學(xué)研究”提供了良好條件,是當(dāng)代電鏡、解剖、組胚、生理、醫(yī)院、化工等實(shí)驗(yàn)系統(tǒng)zui理想的快速制樣理想切片儀器。切片機(jī)經(jīng)過科學(xué)院上海生理研究所、復(fù)旦大學(xué)、第二軍醫(yī)大學(xué)、北京大學(xué)、北京協(xié)和醫(yī)院、廣州中山大學(xué)等單位使用證明,切片機(jī)切固定的腦和脊髓等組織,zui薄可切到10微米,新鮮的腦、心、腎等組織zui薄可切到30微米,且切片完整,片面光滑,染色均勻,其主要技術(shù)指標(biāo)達(dá)到國外同類產(chǎn)品水平。
In order to avoid the destruction of ice crystal and keep the activity of the sample and the good shape of the cell, we need not freeze or embed the tissue specimen when slicing by the microtome ”And "neurobiological study of spinal cord and brain slice" provides good conditions, which is an ideal instrument for rapid sample preparation of Zui experimental system of electron microscopy, anatomy, embryo formation, physiology, hospital, chemical industry, etc. The slicer has been used by Shanghai Institute of physiology, Fudan University, the Second Military Medical University, Peking University, Peking Union Medical College, Guangzhou Zhongshan University and other units. It has been proved that the slicer can cut the fixed brain and spinal cord and other tissues. The thin Zui can be cut to 10 microns. The thin Zui of fresh brain, heart, kidney and other tissues can be cut to 30 microns. The slicer is complete, one-sided and smooth, with uniform staining The main technical indicators have reached the level of similar products abroad.
通常來講,切片機(jī)的操作闡明包含開機(jī)和切片這兩部分。開啟切片機(jī)主機(jī)電源,翻開開關(guān),運(yùn)用箱體溫度設(shè)置按鈕設(shè)置切片時(shí)所需的溫度,這時(shí)溫度顯現(xiàn)窗口會(huì)同步顯現(xiàn)設(shè)置的溫度。設(shè)置好后,過5秒后,會(huì)顯現(xiàn)實(shí)踐溫度。同時(shí),咱們也能夠隨時(shí)查看設(shè)置的溫度巨細(xì)。*次開機(jī)時(shí),切片機(jī)應(yīng)經(jīng)過基準(zhǔn)時(shí)刻設(shè)置按鈕設(shè)置一個(gè)基準(zhǔn)時(shí)刻,經(jīng)過除霜時(shí)刻設(shè)置按鈕來設(shè)置除霜時(shí)刻。假如需求手動(dòng)除霜,則應(yīng)先按下手動(dòng)除霜按鈕,聽到峰鳴音后,按下疾速制冷按鈕可進(jìn)行冷凍臺(tái)的除霜,按下箱體溫度設(shè)置按鈕則是箱體除霜。如要關(guān)閉,只要按此次序再按一次就能夠了。
Generally speaking, the operation of the slicer includes two parts: startup and slicing. Turn on the main power of the slicer, turn on the switch, and use the box temperature setting button to set the required temperature for slicing. At this time, the temperature display window will synchronously display the set temperature. After setting, after 5 seconds, the practice temperature will appear. At the same time, we can check the temperature at any time. *During the secondary startup, the slicer shall set a reference time through the setting button of the reference time, and set the defrosting time through the setting button of the defrosting time. If manual defrosting is required, press the manual defrosting button first. After hearing the peak sound, press the fast cooling button to defrost the freezer. Press the box temperature setting button to defrost the box. To close, just press again in this order.
切片機(jī)能夠運(yùn)用厚度調(diào)理旋鈕來調(diào)理切片厚度,裝置刀片時(shí)應(yīng)調(diào)整好切片角度。切片前,先將樣品進(jìn)行冷卻,要冷卻透徹,然后放到切片機(jī)上,樣品要接近刀口,并調(diào)整好平面。以后按下按鈕,進(jìn)行切片,切出的樣品要平坦的進(jìn)入防卷板與刀片之間的空地,zui終取出,進(jìn)行染色觀察。想要避免切片機(jī)的參數(shù)被更改,能夠?qū)㈡I盤確定,經(jīng)過控制面板上的鍵盤確定按鈕。按下按鈕5秒鐘左右,當(dāng)時(shí)刻窗口中的兩個(gè)點(diǎn)中止閃耀時(shí),就表明鍵盤現(xiàn)已被確定了,想要解鎖辦法同上。切片機(jī)再次開機(jī)前,應(yīng)查看切片機(jī)內(nèi)是不是有積水,如有應(yīng)處理潔凈后再開機(jī),假如需求長時(shí)刻運(yùn)用,那么可將其夜間的溫度調(diào)零下十度以下,這么有利于維護(hù)切片機(jī)。
The slicer can use the thickness adjusting knob to adjust the slice thickness, and the slice angle should be adjusted when the blade is installed. Before slicing, first cool the sample thoroughly, and then put it on the slicer. The sample should be close to the knife edge, and the plane should be adjusted. After that, press the button to slice. The cut sample should enter the open space between the anti roll plate and the blade, and Zui finally takes it out for dyeing observation. To avoid the parameters of the slicer being changed, the keyboard can be determined through the keyboard determination button on the control panel. Press the button for about 5 seconds, when two points in the window stop flashing, it means that the keyboard has been determined, and the way to unlock is the same as above. Before the slicer is turned on again, check whether there is water in the slicer. If there is water in the slicer, clean it and then turn it on again. If it needs to be used for a long time, then adjust its temperature at night to below - 10 ℃, which is conducive to maintaining the slicer.
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